YSO Agar Size:250g respectively 10% and 5% of

respectively 10% and 5% of the NaCl concentration is present in both formulations

Dextrose 30 Casamino acids 2 Agar 17 L-Histidine 0

Folic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin

coli and S

Tryptone 10 NaCl 8 Agar 15 Final pH 7

YSO Agar Size:250g respectively 10% and 5% ofFormula g l Dextrose 30 Casamino acids 2 Agar 17 L Histidine 0. 05 L Leucine 0. 05 L Lysine HCl 0. 05 Uracil 0. 05 Total 49. 20 Suspend 49. 20 gram powdered medium in 1 litre distilled water Store dry at room temperature.