Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calcium

Addition of magnesium and calcium increases the growth of the recombinant cells

With a pKa of 7

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen

95g in 1L of Distilled or De-ionised Water

These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calciumSodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room