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16 pH (100 g/l) 4
coli is grown to late log phase in LB Broth
Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium
EMM containing 0
Methionine
Neidhardt Basal Salt Mixture, Glucose 20 g/L Size:658g (20L) 16 pH (100 g/l) 4Containing all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 32. 9 grams l with Neidhardt supplement mixture in a concentration of 3. 1 grams l. Suspend 32. 9 grams of Neidhardt basal salt mixture plus 3. 1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this
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