Yeast Nitrogen base without Amino acids and without Copper Size:500g HL5-C supplemented with vitamins and

HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media

Kanamycin is used as a selective agent in combination with the incorporation of the NPT II (AH3’) gene in plant tissue and microbial cells

Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor

typically 10%

5 Agar 15 Final pH 7

Yeast Nitrogen base without Amino acids and without Copper Size:500g HL5-C supplemented with vitamins andCupric sulphate, CuSO4. 5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature