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AX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells
M9 Minimal Salts Base
1 Minerals NH4Cl 53
2 at 25°C Suspend 13 gram powdered medium in 1 litre distilled water
05 Total 30
Yeast Potassium Nitrate Nitrogen Base Size:500g AX Medium is used toYeast Potassium Nitrate Nitrogen Base contains Potassium Nitrate as a sole nitrogen source to test nitrogen assimilation. The addition of a carbon source is required. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base. Yeasts which have grown on a rich medium like Yeast Agar may carry a reserve of nitrogen in the form of protein. To avoid errors due to this
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