AX Medium Size:6 x 1kg respectively 10% and 5% of

respectively 10% and 5% of the NaCl concentration is present in both formulations

minus Cysteine and without Lysine

The use of MOPS Buffer allows proteins to run slower than when using MES buffer

Experiments in molecular genetics

3 gram powdered medium in 1 litre distilled water

AX Medium Size:6 x 1kg respectively 10% and 5% ofAX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells. Component g l Peptone 14. 3 Yeast Extract 7. 15 Glucose 18 KH2PO4 0. 49 Na2HPO4. anhydrous 0. 49 Suspend 40. 4 gram powdered medium in 1 litre distilled water. Store dry at room temperature